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#437957 Meet Assembloids, Mini Human Brains With ...
It’s not often that a twitching, snowman-shaped blob of 3D human tissue makes someone’s day.
But when Dr. Sergiu Pasca at Stanford University witnessed the tiny movement, he knew his lab had achieved something special. You see, the blob was evolved from three lab-grown chunks of human tissue: a mini-brain, mini-spinal cord, and mini-muscle. Each individual component, churned to eerie humanoid perfection inside bubbling incubators, is already a work of scientific genius. But Pasca took the extra step, marinating the three components together inside a soup of nutrients.
The result was a bizarre, Lego-like human tissue that replicates the basic circuits behind how we decide to move. Without external prompting, when churned together like ice cream, the three ingredients physically linked up into a fully functional circuit. The 3D mini-brain, through the information highway formed by the artificial spinal cord, was able to make the lab-grown muscle twitch on demand.
In other words, if you think isolated mini-brains—known formally as brain organoids—floating in a jar is creepy, upgrade your nightmares. The next big thing in probing the brain is assembloids—free-floating brain circuits—that now combine brain tissue with an external output.
The end goal isn’t to freak people out. Rather, it’s to recapitulate our nervous system, from input to output, inside the controlled environment of a Petri dish. An autonomous, living brain-spinal cord-muscle entity is an invaluable model for figuring out how our own brains direct the intricate muscle movements that allow us stay upright, walk, or type on a keyboard.
It’s the nexus toward more dexterous brain-machine interfaces, and a model to understand when brain-muscle connections fail—as in devastating conditions like Lou Gehrig’s disease or Parkinson’s, where people slowly lose muscle control due to the gradual death of neurons that control muscle function. Assembloids are a sort of “mini-me,” a workaround for testing potential treatments on a simple “replica” of a person rather than directly on a human.
From Organoids to Assembloids
The miniature snippet of the human nervous system has been a long time in the making.
It all started in 2014, when Dr. Madeleine Lancaster, then a post-doc at Stanford, grew a shockingly intricate 3D replica of human brain tissue inside a whirling incubator. Revolutionarily different than standard cell cultures, which grind up brain tissue to reconstruct as a flat network of cells, Lancaster’s 3D brain organoids were incredibly sophisticated in their recapitulation of the human brain during development. Subsequent studies further solidified their similarity to the developing brain of a fetus—not just in terms of neuron types, but also their connections and structure.
With the finding that these mini-brains sparked with electrical activity, bioethicists increasingly raised red flags that the blobs of human brain tissue—no larger than the size of a pea at most—could harbor the potential to develop a sense of awareness if further matured and with external input and output.
Despite these concerns, brain organoids became an instant hit. Because they’re made of human tissue—often taken from actual human patients and converted into stem-cell-like states—organoids harbor the same genetic makeup as their donors. This makes it possible to study perplexing conditions such as autism, schizophrenia, or other brain disorders in a dish. What’s more, because they’re grown in the lab, it’s possible to genetically edit the mini-brains to test potential genetic culprits in the search for a cure.
Yet mini-brains had an Achilles’ heel: not all were made the same. Rather, depending on the region of the brain that was reverse engineered, the cells had to be persuaded by different cocktails of chemical soups and maintained in isolation. It was a stark contrast to our own developing brains, where regions are connected through highways of neural networks and work in tandem.
Pasca faced the problem head-on. Betting on the brain’s self-assembling capacity, his team hypothesized that it might be possible to grow different mini-brains, each reflecting a different brain region, and have them fuse together into a synchronized band of neuron circuits to process information. Last year, his idea paid off.
In one mind-blowing study, his team grew two separate portions of the brain into blobs, one representing the cortex, the other a deeper part of the brain known to control reward and movement, called the striatum. Shockingly, when put together, the two blobs of human brain tissue fused into a functional couple, automatically establishing neural highways that resulted in one of the most sophisticated recapitulations of a human brain. Pasca crowned this tissue engineering crème-de-la-crème “assembloids,” a portmanteau between “assemble” and “organoids.”
“We have demonstrated that regionalized brain spheroids can be put together to form fused structures called brain assembloids,” said Pasca at the time.” [They] can then be used to investigate developmental processes that were previously inaccessible.”
And if that’s possible for wiring up a lab-grown brain, why wouldn’t it work for larger neural circuits?
Assembloids, Assemble
The new study is the fruition of that idea.
The team started with human skin cells, scraped off of eight healthy people, and transformed them into a stem-cell-like state, called iPSCs. These cells have long been touted as the breakthrough for personalized medical treatment, before each reflects the genetic makeup of its original host.
Using two separate cocktails, the team then generated mini-brains and mini-spinal cords using these iPSCs. The two components were placed together “in close proximity” for three days inside a lab incubator, gently floating around each other in an intricate dance. To the team’s surprise, under the microscope using tracers that glow in the dark, they saw highways of branches extending from one organoid to the other like arms in a tight embrace. When stimulated with electricity, the links fired up, suggesting that the connections weren’t just for show—they’re capable of transmitting information.
“We made the parts,” said Pasca, “but they knew how to put themselves together.”
Then came the ménage à trois. Once the mini-brain and spinal cord formed their double-decker ice cream scoop, the team overlaid them onto a layer of muscle cells—cultured separately into a human-like muscular structure. The end result was a somewhat bizarre and silly-looking snowman, made of three oddly-shaped spherical balls.
Yet against all odds, the brain-spinal cord assembly reached out to the lab-grown muscle. Using a variety of tools, including measuring muscle contraction, the team found that this utterly Frankenstein-like snowman was able to make the muscle component contract—in a way similar to how our muscles twitch when needed.
“Skeletal muscle doesn’t usually contract on its own,” said Pasca. “Seeing that first twitch in a lab dish immediately after cortical stimulation is something that’s not soon forgotten.”
When tested for longevity, the contraption lasted for up to 10 weeks without any sort of breakdown. Far from a one-shot wonder, the isolated circuit worked even better the longer each component was connected.
Pasca isn’t the first to give mini-brains an output channel. Last year, the queen of brain organoids, Lancaster, chopped up mature mini-brains into slices, which were then linked to muscle tissue through a cultured spinal cord. Assembloids are a step up, showing that it’s possible to automatically sew multiple nerve-linked structures together, such as brain and muscle, sans slicing.
The question is what happens when these assembloids become more sophisticated, edging ever closer to the inherent wiring that powers our movements. Pasca’s study targets outputs, but what about inputs? Can we wire input channels, such as retinal cells, to mini-brains that have a rudimentary visual cortex to process those examples? Learning, after all, depends on examples of our world, which are processed inside computational circuits and delivered as outputs—potentially, muscle contractions.
To be clear, few would argue that today’s mini-brains are capable of any sort of consciousness or awareness. But as mini-brains get increasingly more sophisticated, at what point can we consider them a sort of AI, capable of computation or even something that mimics thought? We don’t yet have an answer—but the debates are on.
Image Credit: christitzeimaging.com / Shutterstock.com Continue reading
#437955 Pandemic’s robot ...
Robots that helped people survive and stay safe over the past year are touting their value at the tech industry's annual extravaganza amid a pandemic which has given fresh momentum to the robotics sector. Continue reading
#437805 Video Friday: Quadruped Robot HyQ ...
Video Friday is your weekly selection of awesome robotics videos, collected by your Automaton bloggers. We’ll also be posting a weekly calendar of upcoming robotics events for the next few months; here’s what we have so far (send us your events!):
RSS 2020 – July 12-16, 2020 – [Virtual Conference]
CLAWAR 2020 – August 24-26, 2020 – [Virtual Conference]
ICUAS 2020 – September 1-4, 2020 – Athens, Greece
ICRES 2020 – September 28-29, 2020 – Taipei, Taiwan
IROS 2020 – October 25-29, 2020 – Las Vegas, Nevada
ICSR 2020 – November 14-16, 2020 – Golden, Colorado
Let us know if you have suggestions for next week, and enjoy today’s videos.
Four-legged HyQ balancing on two legs. Nice results from the team at IIT’s Dynamic Legged Systems Lab. And we can’t wait to see the “ninja walk,” currently shown in simulation, implemented with the real robot!
The development of balance controllers for legged robots with point feet remains a challenge when they have to traverse extremely constrained environments. We present a balance controller that has the potential to achieve line walking for quadruped robots. Our initial experiments show the 90-kg robot HyQ balancing on two feet and recovering from external pushes, as well as some changes in posture achieved without losing balance.
[ IIT ]
Thanks Victor!
Ava Robotics’ telepresence robot has been beheaded by MIT, and it now sports a coronavirus-destroying UV array.
UV-C light has proven to be effective at killing viruses and bacteria on surfaces and aerosols, but it’s unsafe for humans to be exposed. Fortunately, Ava’s telepresence robot doesn’t require any human supervision. Instead of the telepresence top, the team subbed in a UV-C array for disinfecting surfaces. Specifically, the array uses short-wavelength ultraviolet light to kill microorganisms and disrupt their DNA in a process called ultraviolet germicidal irradiation. The complete robot system is capable of mapping the space — in this case, GBFB’s warehouse — and navigating between waypoints and other specified areas. In testing the system, the team used a UV-C dosimeter, which confirmed that the robot was delivering the expected dosage of UV-C light predicted by the model.
[ MIT ]
While it’s hard enough to get quadrupedal robots to walk in complex environments, this work from the Robotic Systems Lab at ETH Zurich shows some impressive whole body planning that allows ANYmal to squeeze its body through small or weirdly shaped spaces.
[ RSL ]
Engineering researchers at North Carolina State University and Temple University have developed soft robots inspired by jellyfish that can outswim their real-life counterparts. More practically, the new jellyfish-bots highlight a technique that uses pre-stressed polymers to make soft robots more powerful.
The researchers also used the technique to make a fast-moving robot that resembles a larval insect curling its body, then jumping forward as it quickly releases its stored energy. Lastly, the researchers created a three-pronged gripping robot – with a twist. Most grippers hang open when “relaxed,” and require energy to hold on to their cargo as it is lifted and moved from point A to point B. But this claw’s default position is clenched shut. Energy is required to open the grippers, but once they’re in position, the grippers return to their “resting” mode – holding their cargo tight.
[ NC State ]
As control skills increase, we are more and more impressed by what a Cassie bipedal robot can do. Those who have been following our channel, know that we always show the limitations of our work. So while there is still much to do, you gotta like the direction things are going. Later this year, you will see this controller integrated with our real-time planner and perception system. Autonomy with agility! Watch out for us!
[ University of Michigan ]
GITAI’s S1 arm is a little less exciting than their humanoid torso, but it looks like this one might actually be going to the ISS next year.
Here’s how the humanoid would handle a similar task:
[ GITAI ]
Thanks Fan!
If you need a robot that can lift 250 kg at 10 m/s across a workspace of a thousand cubic meters, here’s your answer.
[ Fraunhofer ]
Penn engineers with funding from the National Science Foundation, have nanocardboard plates able to levitate when bright light is shone on them. This fleet of tiny aircraft could someday explore the skies of other worlds, including Mars. The thinner atmosphere there would give the flyers a boost, enabling them to carry payloads ten times as massive as they are, making them an efficient, light-weight alternative to the Mars helicopter.
[ UPenn ]
Erin Sparks, assistant professor in Plant and Soil Sciences, dreamed of a robot she could use in her research. A perfect partnership was formed when Adam Stager, then a mechanical engineering Ph.D. student, reached out about a robot he had a gut feeling might be useful in agriculture. The pair moved forward with their research with corn at the UD Farm, using the robot to capture dynamic phenotyping information of brace roots over time.
[ Sparks Lab ]
This is a video about robot spy turtles but OMG that bird drone landing gear.
[ PBS ]
If you have a DJI Mavic, you now have something new to worry about.
[ DroGone ]
I was able to spot just one single person in the warehouse footage in this video.
[ Berkshire Grey ]
Flyability has partnered with the ROBINS Project to help fill gaps in the technology used in ship inspections. Watch this video to learn more about the ROBINS project and how Flyability’s drones for confined spaces are helping make inspections on ships safer, cheaper, and more efficient.
[ Flyability ]
In this video, a mission of the Alpha Aerial Scout of Team CERBERUS during the DARPA Subterranean Challenge Urban Circuit event is presented. The Alpha Robot operates inside the Satsop Abandoned Power Plant and performs autonomous exploration. This deployment took place during the 3rd field trial of team CERBERUS during the Urban Circuit event of the DARPA Subterranean Challenge.
[ ARL ]
More excellent talks from the remote Legged Robots ICRA workshop- we’ve posted three here, but there are several other good talks this week as well.
[ ICRA 2020 Legged Robots Workshop ] Continue reading