Tag Archives: muscle
#437957 Meet Assembloids, Mini Human Brains With ...
It’s not often that a twitching, snowman-shaped blob of 3D human tissue makes someone’s day.
But when Dr. Sergiu Pasca at Stanford University witnessed the tiny movement, he knew his lab had achieved something special. You see, the blob was evolved from three lab-grown chunks of human tissue: a mini-brain, mini-spinal cord, and mini-muscle. Each individual component, churned to eerie humanoid perfection inside bubbling incubators, is already a work of scientific genius. But Pasca took the extra step, marinating the three components together inside a soup of nutrients.
The result was a bizarre, Lego-like human tissue that replicates the basic circuits behind how we decide to move. Without external prompting, when churned together like ice cream, the three ingredients physically linked up into a fully functional circuit. The 3D mini-brain, through the information highway formed by the artificial spinal cord, was able to make the lab-grown muscle twitch on demand.
In other words, if you think isolated mini-brains—known formally as brain organoids—floating in a jar is creepy, upgrade your nightmares. The next big thing in probing the brain is assembloids—free-floating brain circuits—that now combine brain tissue with an external output.
The end goal isn’t to freak people out. Rather, it’s to recapitulate our nervous system, from input to output, inside the controlled environment of a Petri dish. An autonomous, living brain-spinal cord-muscle entity is an invaluable model for figuring out how our own brains direct the intricate muscle movements that allow us stay upright, walk, or type on a keyboard.
It’s the nexus toward more dexterous brain-machine interfaces, and a model to understand when brain-muscle connections fail—as in devastating conditions like Lou Gehrig’s disease or Parkinson’s, where people slowly lose muscle control due to the gradual death of neurons that control muscle function. Assembloids are a sort of “mini-me,” a workaround for testing potential treatments on a simple “replica” of a person rather than directly on a human.
From Organoids to Assembloids
The miniature snippet of the human nervous system has been a long time in the making.
It all started in 2014, when Dr. Madeleine Lancaster, then a post-doc at Stanford, grew a shockingly intricate 3D replica of human brain tissue inside a whirling incubator. Revolutionarily different than standard cell cultures, which grind up brain tissue to reconstruct as a flat network of cells, Lancaster’s 3D brain organoids were incredibly sophisticated in their recapitulation of the human brain during development. Subsequent studies further solidified their similarity to the developing brain of a fetus—not just in terms of neuron types, but also their connections and structure.
With the finding that these mini-brains sparked with electrical activity, bioethicists increasingly raised red flags that the blobs of human brain tissue—no larger than the size of a pea at most—could harbor the potential to develop a sense of awareness if further matured and with external input and output.
Despite these concerns, brain organoids became an instant hit. Because they’re made of human tissue—often taken from actual human patients and converted into stem-cell-like states—organoids harbor the same genetic makeup as their donors. This makes it possible to study perplexing conditions such as autism, schizophrenia, or other brain disorders in a dish. What’s more, because they’re grown in the lab, it’s possible to genetically edit the mini-brains to test potential genetic culprits in the search for a cure.
Yet mini-brains had an Achilles’ heel: not all were made the same. Rather, depending on the region of the brain that was reverse engineered, the cells had to be persuaded by different cocktails of chemical soups and maintained in isolation. It was a stark contrast to our own developing brains, where regions are connected through highways of neural networks and work in tandem.
Pasca faced the problem head-on. Betting on the brain’s self-assembling capacity, his team hypothesized that it might be possible to grow different mini-brains, each reflecting a different brain region, and have them fuse together into a synchronized band of neuron circuits to process information. Last year, his idea paid off.
In one mind-blowing study, his team grew two separate portions of the brain into blobs, one representing the cortex, the other a deeper part of the brain known to control reward and movement, called the striatum. Shockingly, when put together, the two blobs of human brain tissue fused into a functional couple, automatically establishing neural highways that resulted in one of the most sophisticated recapitulations of a human brain. Pasca crowned this tissue engineering crème-de-la-crème “assembloids,” a portmanteau between “assemble” and “organoids.”
“We have demonstrated that regionalized brain spheroids can be put together to form fused structures called brain assembloids,” said Pasca at the time.” [They] can then be used to investigate developmental processes that were previously inaccessible.”
And if that’s possible for wiring up a lab-grown brain, why wouldn’t it work for larger neural circuits?
Assembloids, Assemble
The new study is the fruition of that idea.
The team started with human skin cells, scraped off of eight healthy people, and transformed them into a stem-cell-like state, called iPSCs. These cells have long been touted as the breakthrough for personalized medical treatment, before each reflects the genetic makeup of its original host.
Using two separate cocktails, the team then generated mini-brains and mini-spinal cords using these iPSCs. The two components were placed together “in close proximity” for three days inside a lab incubator, gently floating around each other in an intricate dance. To the team’s surprise, under the microscope using tracers that glow in the dark, they saw highways of branches extending from one organoid to the other like arms in a tight embrace. When stimulated with electricity, the links fired up, suggesting that the connections weren’t just for show—they’re capable of transmitting information.
“We made the parts,” said Pasca, “but they knew how to put themselves together.”
Then came the ménage à trois. Once the mini-brain and spinal cord formed their double-decker ice cream scoop, the team overlaid them onto a layer of muscle cells—cultured separately into a human-like muscular structure. The end result was a somewhat bizarre and silly-looking snowman, made of three oddly-shaped spherical balls.
Yet against all odds, the brain-spinal cord assembly reached out to the lab-grown muscle. Using a variety of tools, including measuring muscle contraction, the team found that this utterly Frankenstein-like snowman was able to make the muscle component contract—in a way similar to how our muscles twitch when needed.
“Skeletal muscle doesn’t usually contract on its own,” said Pasca. “Seeing that first twitch in a lab dish immediately after cortical stimulation is something that’s not soon forgotten.”
When tested for longevity, the contraption lasted for up to 10 weeks without any sort of breakdown. Far from a one-shot wonder, the isolated circuit worked even better the longer each component was connected.
Pasca isn’t the first to give mini-brains an output channel. Last year, the queen of brain organoids, Lancaster, chopped up mature mini-brains into slices, which were then linked to muscle tissue through a cultured spinal cord. Assembloids are a step up, showing that it’s possible to automatically sew multiple nerve-linked structures together, such as brain and muscle, sans slicing.
The question is what happens when these assembloids become more sophisticated, edging ever closer to the inherent wiring that powers our movements. Pasca’s study targets outputs, but what about inputs? Can we wire input channels, such as retinal cells, to mini-brains that have a rudimentary visual cortex to process those examples? Learning, after all, depends on examples of our world, which are processed inside computational circuits and delivered as outputs—potentially, muscle contractions.
To be clear, few would argue that today’s mini-brains are capable of any sort of consciousness or awareness. But as mini-brains get increasingly more sophisticated, at what point can we consider them a sort of AI, capable of computation or even something that mimics thought? We don’t yet have an answer—but the debates are on.
Image Credit: christitzeimaging.com / Shutterstock.com Continue reading
#437869 Video Friday: Japan’s Gundam Robot ...
Video Friday is your weekly selection of awesome robotics videos, collected by your Automaton bloggers. We’ll also be posting a weekly calendar of upcoming robotics events for the next few months; here’s what we have so far (send us your events!):
ACRA 2020 – December 8-10, 2020 – [Online]
Let us know if you have suggestions for next week, and enjoy today’s videos.
Another BIG step for Japan’s Gundam project.
[ Gundam Factory ]
We present an interactive design system that allows users to create sculpting styles and fabricate clay models using a standard 6-axis robot arm. Given a general mesh as input, the user iteratively selects sub-areas of the mesh through decomposition and embeds the design expression into an initial set of toolpaths by modifying key parameters that affect the visual appearance of the sculpted surface finish. We demonstrate the versatility of our approach by designing and fabricating different sculpting styles over a wide range of clay models.
[ Disney Research ]
China’s Chang’e-5 completed the drilling, sampling and sealing of lunar soil at 04:53 BJT on Wednesday, marking the first automatic sampling on the Moon, the China National Space Administration (CNSA) announced Wednesday.
[ CCTV ]
Red Hat’s been putting together an excellent documentary on Willow Garage and ROS, and all five parts have just been released. We posted Part 1 a little while ago, so here’s Part 2 and Part 3.
Parts 4 and 5 are at the link below!
[ Red Hat ]
Congratulations to ANYbotics on a well-deserved raise!
ANYbotics has origins in the Robotic Systems Lab at ETH Zurich, and ANYmal’s heritage can be traced back at least as far as StarlETH, which we first met at ICRA 2013.
[ ANYbotics ]
Most conventional robots are working with 0.05-0.1mm accuracy. Such accuracy requires high-end components like low-backlash gears, high-resolution encoders, complicated CNC parts, powerful motor drives, etc. Those in combination end up an expensive solution, which is either unaffordable or unnecessary for many applications. As a result, we found the Apicoo Robotics to provide our customers solutions with a much lower cost and higher stability.
[ Apicoo Robotics ]
The Skydio 2 is an incredible drone that can take incredible footage fully autonomously, but it definitely helps if you do incredible things in incredible places.
[ Skydio ]
Jueying is the first domestic sensitive quadruped robot for industry applications and scenarios. It can coordinate (replace) humans to reach any place that can be reached. It has superior environmental adaptability, excellent dynamic balance capabilities and precise Environmental perception capabilities. By carrying functional modules for different application scenarios in the safe load area, the mobile superiority of the quadruped robot can be organically integrated with the commercialization of functional modules, providing smart factories, smart parks, scene display and public safety application solutions.
[ DeepRobotics ]
We have developed semi-autonomous quadruped robot, called LASER-D (Legged-Agile-Smart-Efficient Robot for Disinfection) for performing disinfection in cluttered environments. The robot is equipped with a spray-based disinfection system and leverages the body motion to controlling the spray action without the need for an extra stabilization mechanism. The system includes an image processing capability to verify disinfected regions with high accuracy. This system allows the robot to successfully carry out effective disinfection tasks while safely traversing through cluttered environments, climb stairs/slopes, and navigate on slippery surfaces.
[ USC Viterbi ]
We propose the “multi-vision hand”, in which a number of small high-speed cameras are mounted on the robot hand of a common 7 degrees-of-freedom robot. Also, we propose visual-servoing control by using a multi-vision system that combines the multi-vision hand and external fixed high-speed cameras. The target task was ball catching motion, which requires high-speed operation. In the proposed catching control, the catch position of the ball, which is estimated by the external fixed high-speed cameras, is corrected by the multi-vision hand in real-time.
More details available through IROS on-demand.
[ Namiki Laboratory ]
Shunichi Kurumaya wrote in to share his work on PneuFinger, a pneumatically actuated compliant robotic gripping system.
[ Nakamura Lab ]
Thanks Shunichi!
Motivated by insights into the human teaching process, we introduce a method for incorporating unstructured natural language into imitation learning. At training time, the expert can provide demonstrations along with verbal descriptions in order to describe the underlying intent, e.g., “Go to the large green bowl’’. The training process, then, interrelates the different modalities to encode the correlations between language, perception, and motion. The resulting language-conditioned visuomotor policies can be conditioned at run time on new human commands and instructions, which allows for more fine-grained control over the trained policies while also reducing situational ambiguity.
[ ASU ]
Thanks Heni!
Gita is on sale for the holidays for only $2,000.
[ Gita ]
This video introduces a computational approach for routing thin artificial muscle actuators through hyperelastic soft robots, in order to achieve a desired deformation behavior. Provided with a robot design, and a set of example deformations, we continuously co-optimize the routing of actuators, and their actuation, to approximate example deformations as closely as possible.
[ Disney Research ]
Researchers and mountain rescuers in Switzerland are making huge progress in the field of autonomous drones as the technology becomes more in-demand for global search-and-rescue operations.
[ SWI ]
This short clip of the Ghost Robotics V60 features an interesting, if awkward looking, righting behavior at the end.
[ Ghost Robotics ]
Europe’s Rosalind Franklin ExoMars rover has a younger ’sibling’, ExoMy. The blueprints and software for this mini-version of the full-size Mars explorer are available for free so that anyone can 3D print, assemble and program their own ExoMy.
[ ESA ]
The holiday season is here, and with the added impact of Covid-19 consumer demand is at an all-time high. Berkshire Grey is the partner that today’s leading organizations turn to when it comes to fulfillment automation.
[ Berkshire Grey ]
Until very recently, the vast majority of studies and reports on the use of cargo drones for public health were almost exclusively focused on the technology. The driving interest from was on the range that these drones could travel, how much they could carry and how they worked. Little to no attention was placed on the human side of these projects. Community perception, community engagement, consent and stakeholder feedback were rarely if ever addressed. This webinar presents the findings from a very recent study that finally sheds some light on the human side of drone delivery projects.
[ WeRobotics ] Continue reading