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#431175 Servosila introduces Mobile Robots ...

Servosila introduces a new member of the family of Servosila “Engineer” robots, a UGV called “Radio Engineer”. This new variant of the well-known backpack-transportable robot features a Software Defined Radio (SDR) payload module integrated into the robotic vehicle.

“Several of our key customers had asked us to enable an Electronic Warfare (EW) or Cognitive Radio applications in our robots”, – says a spokesman for the company, “By integrating a Software Defined Radio (SDR) module into our robotic platforms we cater to both requirements. Radio spectrum analysis, radio signal detection, jamming, and radio relay are important features for EOD robots such as ours. Servosila continues to serve the customers by pushing the boundaries of what their Servosila robots can do. Our partners in the research world and academia shall also greatly benefit from the new functionality that gives them more means of achieving their research goals.”
Photo Credit: Servosila – www.servosila.com
Coupling a programmable mobile robot with a software-defined radio creates a powerful platform for developing innovative applications that mix mobility and artificial intelligence with modern radio technologies. The new robotic radio applications include localized frequency hopping pattern analysis, OFDM waveform recognition, outdoor signal triangulation, cognitive mesh networking, automatic area search for radio emitters, passive or active mobile robotic radars, mobile base stations, mobile radio scanners, and many others.

A rotating head of the robot with mounts for external antennae acts as a pan-and-tilt device thus enabling various scanning and tracking applications. The neck of the robotic head is equipped with a pair of highly accurate Servosila-made servos with a pointing precision of 3.0 angular minutes. This means that the robot can point its antennae with an unprecedented accuracy.

Researchers and academia can benefit from the platform’s support for GnuRadio, an open source software framework for developing SDR applications. An on-board Intel i7 computer capable of executing OpenCL code, is internally connected to the SDR payload module. This makes it possible to execute most existing GnuRadio applications directly on the robot’s on-board computer. Other sensors of the robot such as a GPS sensor, an IMU or a thermal vision camera contribute into sensor fusion algorithms.

Since Servosila “Engineer” mobile robots are primarily designed for outdoor use, the SDR module is fully enclosed into a hardened body of the robot which provides protection in case of dust, rain, snow or impacts with obstacles while the robot is on the move. The robot and its SDR payload module are both powered by an on-board battery thus making the entire robotic radio platform independent of external power supplies.

Servosila plans to start shipping the SDR-equipped robots to international customers in October, 2017.

Web: https://www.servosila.com
YouTube: https://www.youtube.com/user/servosila/videos

About the Company
Servosila is a robotics technology company that designs, produces and markets a range of mobile robots, robotic arms, servo drives, harmonic reduction gears, robotic control systems as well as software packages that make the robots intelligent. Servosila provides consulting, training and operations support services to various customers around the world. The company markets its products and services directly or through a network of partners who provide tailored and localized services that meet specific procurement, support or operational needs.
Press Release above is by: Servosila
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Posted in Human Robots

#431159 How Close Is Turing’s Dream of ...

The quest for conversational artificial intelligence has been a long one.
When Alan Turing, the father of modern computing, racked his considerable brains for a test that would truly indicate that a computer program was intelligent, he landed on this area. If a computer could convince a panel of human judges that they were talking to a human—if it could hold a convincing conversation—then it would indicate that artificial intelligence had advanced to the point where it was indistinguishable from human intelligence.
This gauntlet was thrown down in 1950 and, so far, no computer program has managed to pass the Turing test.
There have been some very notable failures, however: Joseph Weizenbaum, as early as 1966—when computers were still programmed with large punch-cards—developed a piece of natural language processing software called ELIZA. ELIZA was a machine intended to respond to human conversation by pretending to be a psychotherapist; you can still talk to her today.
Talking to ELIZA is a little strange. She’ll often rephrase things you’ve said back at you: so, for example, if you say “I’m feeling depressed,” she might say “Did you come to me because you are feeling depressed?” When she’s unsure about what you’ve said, ELIZA will usually respond with “I see,” or perhaps “Tell me more.”
For the first few lines of dialogue, especially if you treat her as your therapist, ELIZA can be convincingly human. This was something Weizenbaum noticed and was slightly alarmed by: people were willing to treat the algorithm as more human than it really was. Before long, even though some of the test subjects knew ELIZA was just a machine, they were opening up with some of their deepest feelings and secrets. They were pouring out their hearts to a machine. When Weizenbaum’s secretary spoke to ELIZA, even though she knew it was a fairly simple computer program, she still insisted Weizenbaum leave the room.
Part of the unexpected reaction ELIZA generated may be because people are more willing to open up to a machine, feeling they won’t be judged, even if the machine is ultimately powerless to do or say anything to really help. The ELIZA effect was named for this computer program: the tendency of humans to anthropomorphize machines, or think of them as human.

Weizenbaum himself, who later became deeply suspicious of the influence of computers and artificial intelligence in human life, was astonished that people were so willing to believe his script was human. He wrote, “I had not realized…that extremely short exposures to a relatively simple computer program could induce powerful delusional thinking in quite normal people.”

“Consciously, you know you’re talking to a big block of code stored somewhere out there in the ether. But subconsciously, you might feel like you’re interacting with a human.”

The ELIZA effect may have disturbed Weizenbaum, but it has intrigued and fascinated others for decades. Perhaps you’ve noticed it in yourself, when talking to an AI like Siri, Alexa, or Google Assistant—the occasional response can seem almost too real. Consciously, you know you’re talking to a big block of code stored somewhere out there in the ether. But subconsciously, you might feel like you’re interacting with a human.
Yet the ELIZA effect, as enticing as it is, has proved a source of frustration for people who are trying to create conversational machines. Natural language processing has proceeded in leaps and bounds since the 1960s. Now you can find friendly chatbots like Mitsuku—which has frequently won the Loebner Prize, awarded to the machines that come closest to passing the Turing test—that aim to have a response to everything you might say.
In the commercial sphere, Facebook has opened up its Messenger program and provided software for people and companies to design their own chatbots. The idea is simple: why have an app for, say, ordering pizza when you can just chatter to a robot through your favorite messenger app and make the order in natural language, as if you were telling your friend to get it for you?
Startups like Semantic Machines hope their AI assistant will be able to interact with you just like a secretary or PA would, but with an unparalleled ability to retrieve information from the internet. They may soon be there.
But people who engineer chatbots—both in the social and commercial realm—encounter a common problem: the users, perhaps subconsciously, assume the chatbots are human and become disappointed when they’re not able to have a normal conversation. Frustration with miscommunication can often stem from raised initial expectations.
So far, no machine has really been able to crack the problem of context retention—understanding what’s been said before, referring back to it, and crafting responses based on the point the conversation has reached. Even Mitsuku will often struggle to remember the topic of conversation beyond a few lines of dialogue.

“For everything you say, there could be hundreds of responses that would make sense. When you travel a layer deeper into the conversation, those factors multiply until you end up with vast numbers of potential conversations.”

This is, of course, understandable. Conversation can be almost unimaginably complex. For everything you say, there could be hundreds of responses that would make sense. When you travel a layer deeper into the conversation, those factors multiply until—like possible games of Go or chess—you end up with vast numbers of potential conversations.
But that hasn’t deterred people from trying, most recently, tech giant Amazon, in an effort to make their AI voice assistant, Alexa, friendlier. They have been running the Alexa Prize competition, which offers a cool $500,000 to the winning AI—and a bonus of a million dollars to any team that can create a ‘socialbot’ capable of sustaining a conversation with human users for 20 minutes on a variety of themes.
Topics Alexa likes to chat about include science and technology, politics, sports, and celebrity gossip. The finalists were recently announced: chatbots from universities in Prague, Edinburgh, and Seattle. Finalists were chosen according to the ratings from Alexa users, who could trigger the socialbots into conversation by saying “Hey Alexa, let’s chat,” although the reviews for the socialbots weren’t always complimentary.
By narrowing down the fields of conversation to a specific range of topics, the Alexa Prize has cleverly started to get around the problem of context—just as commercially available chatbots hope to do. It’s much easier to model an interaction that goes a few layers into the conversational topic if you’re limiting those topics to a specific field.
Developing a machine that can hold almost any conversation with a human interlocutor convincingly might be difficult. It might even be a problem that requires artificial general intelligence to truly solve, rather than the previously-employed approaches of scripted answers or neural networks that associate inputs with responses.
But a machine that can have meaningful interactions that people might value and enjoy could be just around the corner. The Alexa Prize winner is announced in November. The ELIZA effect might mean we will relate to machines sooner than we’d thought.
So, go well, little socialbots. If you ever want to discuss the weather or what the world will be like once you guys take over, I’ll be around. Just don’t start a therapy session.
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Posted in Human Robots

#431058 How to Make Your First Chatbot With the ...

You’re probably wondering what Game of Thrones has to do with chatbots and artificial intelligence. Before I explain this weird connection, I need to warn you that this article may contain some serious spoilers. Continue with your reading only if you are a passionate GoT follower, who watches new episodes immediately after they come out.
Why are chatbots so important anyway?
According to the study “When Will AI Exceed Human Performance?,” researchers believe there is a 50% chance artificial intelligence could take over all human jobs by around the year 2060. This technology has already replaced dozens of customer service and sales positions and helped businesses make substantial savings.
Apart from the obvious business advantages, chatbot creation can be fun. You can create an artificial personality with a strong attitude and a unique set of traits and flaws. It’s like creating a new character for your favorite TV show. That’s why I decided to explain the most important elements of the chatbot creation process by using the TV characters we all know and love (or hate).
Why Game of Thrones?
Game of Thrones is the most popular TV show in the world. More than 10 million viewers watched the seventh season premiere, and you have probably seen internet users fanatically discussing the series’ characters, storyline, and possible endings.
Apart from writing about chatbots, I’m also a GoT fanatic, and I will base this chatbot on one of the characters from my favorite series. But before you find out the name of my bot, you should read a few lines about incredible free tools that allow us to build chatbots without coding.
Are chatbots expensive?
Today, you can create a chatbot even if you don’t know how to code. Most chatbot building platforms offer at least one free plan that allows you to use basic functionalities, create your bot, deploy it to Facebook Messenger, and analyze its performance. Free plans usually allow your bot to talk to a limited number of users.
Why should you personalize your bot?
Every platform will ask you to write a bot’s name before you start designing conversations. You will also be able to add the bot’s photograph and bio. Personalizing your bot is the only way to ensure that you will stick to the same personality and storyline throughout the building process. Users often see chatbots as people, and by giving your bot an identity, you will make sure that it doesn’t sound like it has multiple personality disorder.
I think connecting my chatbot with a GoT character will help readers understand the process of chatbot creation.
And the name of our GoT chatbot is…
…Cersei. She is mean, pragmatic, and fearless and she would do anything to stay on the Iron Throne. Many people would rather hang out with Daenerys or Jon Snow. These characters are honest, noble and good-hearted, which means their actions are often predictable.
Cersei, on the other hand, is the queen of intrigues. As the meanest and the most vengeful character in the series, she has an evil plan for everybody who steps on her toes. While viewers can easily guess where Jon and Daenerys stand, there are dozens of questions they would like to ask Cersei. But before we start talking to our bot, we need to build her personality by using the most basic elements of chatbot interaction.
Choosing the bot’s name on Botsify.
Welcome / Greeting Message
The welcome message is the greeting Cersei says to every commoner who clicks on the ‘start conversation’ button. She is not a welcoming person (ask Sansa), except if you are a banker from Braavos. Her introductory message may sound something like this:
“Dear {{user_full_name}}, My name is Cersei of the House Lannister, the First of Her Name, Queen of the Andals and the First Men, Protector of the Seven Kingdoms. You can ask me questions, and I will answer them. If the question is not worth answering, I will redirect you to Ser Gregor Clegane, who will give you a step-by-step course on how to talk to the Queen of Westeros.”
Creating the welcome message on Chatfuel
Default Message / Answer
In the bot game, users, bots, and their creators often need to learn from failed attempts and mistakes. The default message is the text Cersei will send whenever you ask her a question she doesn’t understand. Knowing Cersei, it would sound something like this:
“Ser Gregor, please escort {{user_full_name}} to the dungeon.”
Creating default message on Botsify
Menu
To avoid calling out the Mountain every time someone asks her a question, Cersei might give you a few (safe) options to choose. The best way to do this is by using a menu function. We can classify the questions people want to ask Cersei in several different categories:

Iron Throne
Relationship with Jaime — OK, this isn’t a “safe option,” get ready to get close and personal with Sir Gregor Clegane.
War plans
Euron Greyjoy

After users choose a menu item, Cersei can give them a default response on the topic or set up a plot that will make their lives miserable. Knowing Cersei, she will probably go for the second option.
Adding chatbot menu on Botsify
Stories / Blocks
This feature allows us to build a longer Cersei-to-user interaction. The structure of stories and blocks is different on every chatbot platform, but most of them use keywords and phrases for finding out the user’s intention.

Keywords — where the bot recognizes a certain keyword within the user’s reply. Users who have chosen the ‘war plans’ option might ask Cersei how is she planning to defeat Daenerys’s dragons. We can add ‘dragon’ and ‘dragons’ as keywords, and connect them with an answer that will sound something like this:

“Dragons are not invulnerable as you may think. Maester Qyburn is developing a weapon that will bring them down for good!”
Adding keywords on Chatfuel
People may also ask her about White Walkers. Do you plan to join Daenerys and Jon Snow in a fight against White Walkers? After we add ‘White Walker’ and ‘White Walkers’ on the keyword list, Cersei will answer:
“White Walkers? Do you think the Queen of Westeros has enough free time to think about creatures from fairy tales and legends?”
Adding Keywords on Botsify

Phrases — are more complex syntaxes that the bot can be trained to recognize. Many people would like to ask Cersei if she’s going to marry Euron Greyjoy after the war ends. We can add ‘Euron’ as a keyword, but then we won’t be sure what answer the user is expecting. Instead, we can use the phrase ‘(Will you) marry Euron Greyjoy (after the war?)’. Just to be sure, we should also add a few alternative phrases like ‘(Do you plan on) marrying Euron Greyjoy (after the war),’ ‘(Will you) end up with Euron Greyjoy (after the war?)’, ‘(Will) Euron Greyjoy be the new King?’ etc. Cersei would probably answer this inquiry in her style:

“Of course not, Euron is a useful idiot. I will use his fleet and send him back to the Iron Islands, where he belongs.”
Adding phrases on Botsify
Forms
We have already asked Cersei several questions, and now she would like to ask us something. She can do so by using the form/user input feature. Most tools allow us to add a question and the criteria for checking the users’ answer. If the user provides us the answer that is compliant to the predefined form (like email address, phone number, or a ZIP code), the bot will identify and extract the answer. If the answer doesn’t fit into the predefined criteria, the bot will notify the user and ask him/her to try again.
If Cersei would ask you a question, she would probably want to know your address so she could send her guards to fill your basement with barrels of wildfire.
Creating forms on Botsify
Templates
If you have problems building your first chatbot, templates can help you create the basic conversation structure. Unfortunately, not all platforms offer this feature for free. Snatchbot currently has the most comprehensive list of free templates. There you can choose a pre-built layout. The template selection ranges from simple FAQ bots to ones created for a specific industry, like banking, airline, healthcare, or e-commerce.
Choosing templates on Snatchbot
Plugins
Most tools also provide plugins that can be used for making the conversations more meaningful. These plugins allow Cersei to send images, audio and video files. She can unleash her creativity and make you suffer by sending you her favorite GoT execution videos.

With the help of integrations, Cersei can talk to you on Facebook Messenger, Telegram, WeChat, Slack, and many other communication apps. She can also sell her fan gear and ask you for donations by integrating in-bot payments from PayPal accounts. Her sales pitch will probably sound something like this:
“Gold wins wars! Would you rather invest your funds in a member of a respected family, who always pays her debts, or in the chaotic war endeavor of a crazy revolutionary, whose strength lies in three flying lizards? If your pockets are full of gold, you are already on my side. Now you can complete your checkout on PayPal.”
Chatbot building is now easier than ever, and even small businesses are starting to use the incredible benefits of artificial intelligence. If you still don’t believe that chatbots can replace customer service representatives, I suggest you try to develop a bot based on your favorite TV show, movie or book character and talk with him/her for a while. This way, you will be able to understand the concept that stands behind this amazing technology and use it to improve your business.
Now I’m off to talk to Cersei. Maybe she will feed me some Season 8 spoilers.
This article was originally published by Chatbots Magazine. Read the original post here.
Image credits for screenshots in post: Branislav Srdanovic
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Posted in Human Robots

#430868 These 7 Forces Are Changing the World at ...

It was the Greek philosopher Heraclitus who first said, “The only thing that is constant is change.”
He was onto something. But even he would likely be left speechless at the scale and pace of change the world has experienced in the past 100 years—not to mention the past 10.
Since 1917, the global population has gone from 1.9 billion people to 7.5 billion. Life expectancy has more than doubled in many developing countries and risen significantly in developed countries. In 1917 only eight percent of homes had phones—in the form of landline telephones—while today more than seven in 10 Americans own a smartphone—aka, a supercomputer that fits in their pockets.
And things aren’t going to slow down anytime soon. In a talk at Singularity University’s Global Summit this week in San Francisco, SU cofounder and chairman Peter Diamandis told the audience, “Tomorrow’s speed of change will make today look like we’re crawling.” He then shared his point of view about some of the most important factors driving this accelerating change.
Peter Diamandis at Singularity University’s Global Summit in San Francisco.
Computation
In 1965, Gordon Moore (cofounder of Intel) predicted computer chips would double in power and halve in cost every 18 to 24 months. What became known as Moore’s Law turned out to be accurate, and today affordable computer chips contain a billion or more transistors spaced just nanometers apart.
That means computers can do exponentially more calculations per second than they could thirty, twenty, or ten years ago—and at a dramatically lower cost. This in turn means we can generate a lot more information, and use computers for all kinds of applications they wouldn’t have been able to handle in the past (like diagnosing rare forms of cancer, for example).
Convergence
Increased computing power is the basis for a myriad of technological advances, which themselves are converging in ways we couldn’t have imagined a couple decades ago. As new technologies advance, the interactions between various subsets of those technologies create new opportunities that accelerate the pace of change much more than any single technology can on its own.
A breakthrough in biotechnology, for example, might spring from a crucial development in artificial intelligence. An advance in solar energy could come about by applying concepts from nanotechnology.
Interface Moments
Technology is becoming more accessible even to the most non-techy among us. The internet was once the domain of scientists and coders, but these days anyone can make their own web page, and browsers make those pages easily searchable. Now, interfaces are opening up areas like robotics or 3D printing.
As Diamandis put it, “You don’t need to know how to code to 3D print an attachment for your phone. We’re going from mind to materialization, from intentionality to implication.”
Artificial intelligence is what Diamandis calls “the ultimate interface moment,” enabling everyone who can speak their mind to connect and leverage exponential technologies.
Connectivity
Today there are about three billion people around the world connected to the internet—that’s up from 1.8 billion in 2010. But projections show that by 2025 there will be eight billion people connected. This is thanks to a race between tech billionaires to wrap the Earth in internet; Elon Musk’s SpaceX has plans to launch a network of 4,425 satellites to get the job done, while Google’s Project Loon is using giant polyethylene balloons for the task.
These projects will enable five billion new minds to come online, and those minds will have access to exponential technologies via interface moments.
Sensors
Diamandis predicts that after we establish a 5G network with speeds of 10–100 Gbps, a proliferation of sensors will follow, to the point that there’ll be around 100,000 sensors per city block. These sensors will be equipped with the most advanced AI, and the combination of these two will yield an incredible amount of knowledge.
“By 2030 we’re heading towards 100 trillion sensors,” Diamandis said. “We’re heading towards a world in which we’re going to be able to know anything we want, anywhere we want, anytime we want.” He added that tens of thousands of drones will hover over every major city.
Intelligence
“If you think there’s an arms race going on for AI, there’s also one for HI—human intelligence,” Diamandis said. He explained that if a genius was born in a remote village 100 years ago, he or she would likely not have been able to gain access to the resources needed to put his or her gifts to widely productive use. But that’s about to change.
Private companies as well as military programs are working on brain-machine interfaces, with the ultimate aim of uploading the human mind. The focus in the future will be on increasing intelligence of individuals as well as companies and even countries.
Wealth Concentration
A final crucial factor driving mass acceleration is the increase in wealth concentration. “We’re living in a time when there’s more wealth in the hands of private individuals, and they’re willing to take bigger risks than ever before,” Diamandis said. Billionaires like Mark Zuckerberg, Jeff Bezos, Elon Musk, and Bill Gates are putting millions of dollars towards philanthropic causes that will benefit not only themselves, but humanity at large.
What It All Means
One of the biggest implications of the rate at which the world is changing, Diamandis said, is that the cost of everything is trending towards zero. We are heading towards abundance, and the evidence lies in the reduction of extreme poverty we’ve already seen and will continue to see at an even more rapid rate.
Listening to Diamandis’ optimism, it’s hard not to find it contagious.

“The world is becoming better at an extraordinary rate,” he said, pointing out the rises in literacy, democracy, vaccinations, and life expectancy, and the concurrent decreases in child mortality, birth rate, and poverty.
“We’re alive during a pivotal time in human history,” he concluded. “There is nothing we don’t have access to.”
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#430830 Biocomputers Made From Cells Can Now ...

When it comes to biomolecules, RNA doesn’t get a lot of love.
Maybe you haven’t even heard of the silent workhorse. RNA is the cell’s de facto translator: like a game of telephone, RNA takes DNA’s genetic code to a cellular factory called ribosomes. There, the cell makes proteins based on RNA’s message.
But RNA isn’t just a middleman. It controls what proteins are formed. Because proteins wiz around the cell completing all sorts of important processes, you can say that RNA is the gatekeeper: no RNA message, no proteins, no life.
In a new study published in Nature, RNA finally took center stage. By adding bits of genetic material to the E. Coli bacteria, a team of biohackers at the Wyss Institute hijacked the organism’s RNA messengers so that they only spring into action following certain inputs.
The result? A bacterial biocomputer capable of performing 12-input logic operations—AND, OR, and NOT—following specific inputs. Rather than outputting 0s and 1s, these biocircuits produce results based on the presence or absence of proteins and other molecules.
“It’s the greatest number of inputs in a circuit that a cell has been able to process,” says study author Dr. Alexander Green at Arizona State University. “To be able to analyze those signals and make a decision is the big advance here.”
When given a specific set of inputs, the bacteria spit out a protein that made them glow neon green under fluorescent light.
But synthetic biology promises far more than just a party trick—by tinkering with a cell’s RNA repertoire, scientists may one day coax them to photosynthesize, produce expensive drugs on the fly, or diagnose and hunt down rogue tumor cells.
Illustration of an RNA-based ‘ribocomputing’ device that makes logic-based decisions in living cells. The long gate RNA (blue) detects the binding of an input RNA (red). The ribosome (purple/mauve) reads the gate RNA to produce an output protein. Image Credit: Alexander Green / Arizona State University
The software of life
This isn’t the first time that scientists hijacked life’s algorithms to reprogram cells into nanocomputing systems. Previous work has already introduced to the world yeast cells that can make anti-malaria drugs from sugar or mammalian cells that can perform Boolean logic.
Yet circuits with multiple inputs and outputs remain hard to program. The reason is this: synthetic biologists have traditionally focused on snipping, fusing, or otherwise arranging a cell’s DNA to produce the outcomes they want.
But DNA is two steps removed from proteins, and tinkering with life’s code often leads to unexpected consequences. For one, the cell may not even accept and produce the extra bits of DNA code. For another, the added code, when transformed into proteins, may not act accordingly in the crowded and ever-changing environment of the cell.
What’s more, tinkering with one gene is often not enough to program an entirely new circuit. Scientists often need to amp up or shut down the activity of multiple genes, or multiple biological “modules” each made up of tens or hundreds of genes.
It’s like trying to fit new Lego pieces in a specific order into a room full of Lego constructions. Each new piece has the potential to wander off track and click onto something it’s not supposed to touch.
Getting every moving component to work in sync—as you might have guessed—is a giant headache.
The RNA way
With “ribocomputing,” Green and colleagues set off to tackle a main problem in synthetic biology: predictability.
Named after the “R (ribo)” in “RNA,” the method grew out of an idea that first struck Green back in 2012.
“The synthetic biological circuits to date have relied heavily on protein-based regulators that are difficult to scale up,” Green wrote at the time. We only have a limited handful of “designable parts” that work well, and these circuits require significant resources to encode and operate, he explains.
RNA, in comparison, is a lot more predictable. Like its more famous sibling DNA, RNA is composed of units that come in four different flavors: A, G, C, and U. Although RNA is only single-stranded, rather than the double helix for which DNA is known for, it can bind short DNA-like sequences in a very predictable manner: Gs always match up with Cs and As always with Us.
Because of this predictability, it’s possible to design RNA components that bind together perfectly. In other words, it reduces the chance that added RNA bits might go rogue in an unsuspecting cell.
Normally, once RNA is produced it immediately rushes to the ribosome—the cell’s protein-building factory. Think of it as a constantly “on” system.
However, Green and his team found a clever mechanism to slow them down. Dubbed the “toehold switch,” it works like this: the artificial RNA component is first incorporated into a chain of A, G, C, and U folded into a paperclip-like structure.
This blocks the RNA from accessing the ribosome. Because one RNA strand generally maps to one protein, the switch prevents that protein from ever getting made.
In this way, the switch is set to “off” by default—a “NOT” gate, in Boolean logic.
To activate the switch, the cell needs another component: a “trigger RNA,” which binds to the RNA toehold switch. This flips it on: the RNA grabs onto the ribosome, and bam—proteins.
BioLogic gates
String a few RNA switches together, with the activity of each one relying on the one before, and it forms an “AND” gate. Alternatively, if the activity of each switch is independent, that’s an “OR” gate.
“Basically, the toehold switches performed so well that we wanted to find a way to best exploit them for cellular applications,” says Green. They’re “kind of the equivalent of your first transistors,” he adds.
Once the team optimized the designs for different logic gates, they carefully condensed the switches into “gate RNA” molecules. These gate RNAs contain both codes for proteins and the logic operations needed to kickstart the process—a molecular logic circuit, so to speak.
If you’ve ever played around with an Arduino-controlled electrical circuit, you probably know the easiest way to test its function is with a light bulb.
That’s what the team did here, though with a biological bulb: green fluorescent protein, a light-sensing protein not normally present in bacteria that—when turned on—makes the microbugs glow neon green.
In a series of experiments, Green and his team genetically inserted gate RNAs into bacteria. Then, depending on the type of logical function, they added different combinations of trigger RNAs—the inputs.
When the input RNA matched up with its corresponding gate RNA, it flipped on the switch, causing the cell to light up.

Their most complex circuit contained five AND gates, five OR gates, and two NOTs—a 12-input ribocomputer that functioned exactly as designed.
That’s quite the achievement. “Everything is interacting with everything else and there are a million ways those interactions could flip the switch on accident,” says RNA researcher Dr. Julies Lucks at Northwestern University.
The specificity is thanks to RNA, the authors explain. Because RNAs bind to others so predictably, we can now design massive libraries of gate and trigger units to mix-and-match into all types of nano-biocomputers.
RNA BioNanobots
Although the technology doesn’t have any immediate applications, the team has high hopes.
For the first time, it’s now possible to massively scale up the process of programming new circuits into living cells. We’ve expanded the library of available biocomponents that can be used to reprogram life’s basic code, the authors say.
What’s more, when freeze-dried onto a piece of tissue paper, RNA keeps very well. We could potentially print RNA toehold switches onto paper that respond to viruses or to tumor cells, the authors say, essentially transforming the technology into highly accurate diagnostic platforms.
But Green’s hopes are even wilder for his RNA-based circuits.
“Because we’re using RNA, a universal molecule of life, we know these interactions can also work in other cells, so our method provides a general strategy that could be ported to other organisms,” he says.
Ultimately, the hope is to program neural network-like capabilities into the body’s other cells.
Imagine cells endowed with circuits capable of performing the kinds of computation the brain does, the authors say.
Perhaps one day, synthetic biology will transform our own cells into fully programmable entities, turning us all into biological cyborgs from the inside. How wild would that be?
Image Credit: Wyss Institute at Harvard University Continue reading

Posted in Human Robots