Tag Archives: better
#439077 How Scientists Grew Human Muscles in Pig ...
The little pigs bouncing around the lab looked exceedingly normal. Yet their adorable exterior hid a remarkable secret: each piglet carried two different sets of genes. For now, both sets came from their own species. But one day, one of those sets may be human.
The piglets are chimeras—creatures with intermingled sets of genes, as if multiple entities were seamlessly mashed together. Named after the Greek lion-goat-serpent monsters, chimeras may hold the key to an endless supply of human organs and tissues for transplant. The crux is growing these human parts in another animal—one close enough in size and function to our own.
Last week, a team from the University of Minnesota unveiled two mind-bending chimeras. One was joyous little piglets, each propelled by muscles grown from a different pig. Another was pig embryos, transplanted into surrogate pigs, that developed human muscles for more than 20 days.
The study, led by Drs. Mary and Daniel Garry at the University of Minnesota, had a therapeutic point: engineering a brilliant way to replace muscle loss, especially for the muscles around our skeletons that allow us to move and navigate the world. Trauma and injury, such as from firearm wounds or car crashes, can damage muscle tissue beyond the point of repair. Unfortunately, muscles are also stubborn in that donor tissue from cadavers doesn’t usually “take” at the injury site. For now, there are no effective treatments for severe muscle death, called volumetric muscle loss.
The new human-pig hybrids are designed to tackle this problem. Muscle wasting aside, the study also points to a clever “hack” that increases the amount of human tissue inside a growing pig embryo.
If further improved, the technology could “provide an unlimited supply of organs for transplantation,” said Dr. Mary Garry to Inverse. What’s more, because the human tissue can be sourced from patients themselves, the risk of rejection by the immune system is relatively low—even when grown inside a pig.
“The shortage of organs for heart transplantation, vascular grafting, and skeletal muscle is staggering,” said Garry. Human-animal chimeras could have a “seismic impact” that transforms organ transplantation and helps solve the organ shortage crisis.
That is, if society accepts the idea of a semi-humanoid pig.
Wait…But How?
The new study took a page from previous chimera recipes.
The main ingredients and steps go like this: first, you need an embryo that lacks the ability to develop a tissue or organ. This leaves an “empty slot” of sorts that you can fill with another set of genes—pig, human, or even monkey.
Second, you need to fine-tune the recipe so that the embryos “take” the new genes, incorporating them into their bodies as if they were their own. Third, the new genes activate to instruct the growing embryo to make the necessary tissue or organs without harming the overall animal. Finally, the foreign genes need to stay put, without cells migrating to another body part—say, the brain.
Not exactly straightforward, eh? The piglets are technological wonders that mix cutting-edge gene editing with cloning technologies.
The team went for two chimeras: one with two sets of pig genes, the other with a pig and human mix. Both started with a pig embryo that can’t make its own skeletal muscles (those are the muscles surrounding your bones). Using CRISPR, the gene-editing Swiss Army Knife, they snipped out three genes that are absolutely necessary for those muscles to develop. Like hitting a bullseye with three arrows simultaneously, it’s already a technological feat.
Here’s the really clever part: the muscles around your bones have a slightly different genetic makeup than the ones that line your blood vessels or the ones that pump your heart. While the resulting pig embryos had severe muscle deformities as they developed, their hearts beat as normal. This means the gene editing cut only impacted skeletal muscles.
Then came step two: replacing the missing genes. Using a microneedle, the team injected a fertilized and slightly developed pig egg—called a blastomere—into the embryo. If left on its natural course, a blastomere eventually develops into another embryo. This step “smashes” the two sets of genes together, with the newcomer filling the muscle void. The hybrid embryo was then placed into a surrogate, and roughly four months later, chimeric piglets were born.
Equipped with foreign DNA, the little guys nevertheless seemed totally normal, nosing around the lab and running everywhere without obvious clumsy stumbles. Under the microscope, their “xenomorph” muscles were indistinguishable from run-of-the-mill average muscle tissue—no signs of damage or inflammation, and as stretchy and tough as muscles usually are. What’s more, the foreign DNA seemed to have only developed into muscles, even though they were prevalent across the body. Extensive fishing experiments found no trace of the injected set of genes inside blood vessels or the brain.
A Better Human-Pig Hybrid
Confident in their recipe, the team next repeated the experiment with human cells, with a twist. Instead of using controversial human embryonic stem cells, which are obtained from aborted fetuses, they relied on induced pluripotent stem cells (iPSCs). These are skin cells that have been reverted back into a stem cell state.
Unlike previous attempts at making human chimeras, the team then scoured the genetic landscape of how pig and human embryos develop to find any genetic “brakes” that could derail the process. One gene, TP53, stood out, which was then promptly eliminated with CRISPR.
This approach provides a way for future studies to similarly increase the efficiency of interspecies chimeras, the team said.
The human-pig embryos were then carefully grown inside surrogate pigs for less than a month, and extensively analyzed. By day 20, the hybrids had already grown detectable human skeletal muscle. Similar to the pig-pig chimeras, the team didn’t detect any signs that the human genes had sprouted cells that would eventually become neurons or other non-muscle cells.
For now, human-animal chimeras are not allowed to grow to term, in part to stem the theoretical possibility of engineering humanoid hybrid animals (shudder). However, a sentient human-pig chimera is something that the team specifically addressed. Through multiple experiments, they found no trace of human genes in the embryos’ brain stem cells 20 and 27 days into development. Similarly, human donor genes were absent in cells that would become the hybrid embryos’ reproductive cells.
Despite bioethical quandaries and legal restrictions, human-animal chimeras have taken off, both as a source of insight into human brain development and a well of personalized organs and tissues for transplant. In 2019, Japan lifted its ban on developing human brain cells inside animal embryos, as well as the term limit—to global controversy. There’s also the question of animal welfare, given that hybrid clones will essentially become involuntary organ donors.
As the debates rage on, scientists are nevertheless pushing the limits of human-animal chimeras, while treading as carefully as possible.
“Our data…support the feasibility of the generation of these interspecies chimeras, which will serve as a model for translational research or, one day, as a source for xenotransplantation,” the team said.
Image Credit: Christopher Carson on Unsplash Continue reading
Posted in Human Robots
#439062 Xenobots 2.0: These Living Robots ...
The line between animals and machines was already getting blurry after a team of scientists and roboticists unveiled the first living robots last year. Now the same team has released version 2.0 of their so-called xenobots, and they’re faster, stronger, and more capable than ever.
In January 2020, researchers from Tufts University and the University of Vermont laid out a method for building tiny biological machines out of the eggs of the African claw frog Xenopus laevis. Dubbed xenobots after their animal forebear, they could move independently, push objects, and even team up to create swarms.
Remarkably, building them involved no genetic engineering. Instead, the team used an evolutionary algorithm running on a supercomputer to test out thousands of potential designs made up of different configurations of cells.
Once they’d found some promising candidates that could solve the tasks they were interested in, they used microsurgical tools to build real-world versions out of living cells. The most promising design was built by splicing heart muscle cells (which could contract to propel the xenobots), and skin cells (which provided a rigid support).
Impressive as that might sound, having to build each individual xenobot by hand is obviously tedious. But now the team has devised a new approach that works from the bottom up by getting the xenobots to self-assemble their bodies from single cells. Not only is the approach more scalable, the new xenobots are faster, live longer, and even have a rudimentary memory.
In a paper in Science Robotics, the researchers describe how they took stem cells from frog embryos and allowed them to grow into clumps of several thousand cells called spheroids. After a few days, the stem cells had turned into skin cells covered in small hair-like projections called cilia, which wriggle back and forth.
Normally, these structures are used to spread mucus around on the frog’s skin. But when divorced from their normal context they took on a function more similar to that seen in microorganisms, which use cilia to move about by acting like tiny paddles.
“We are witnessing the remarkable plasticity of cellular collectives, which build a rudimentary new ‘body’ that is quite distinct from their default—in this case, a frog—despite having a completely normal genome,” corresponding author Michael Levin from Tufts University said in a press release.
“We see that cells can re-purpose their genetically encoded hardware, like cilia, for new functions such as locomotion. It is amazing that cells can spontaneously take on new roles and create new body plans and behaviors without long periods of evolutionary selection for those features,” he said.
Not only were the new xenobots faster and longer-lived, they were also much better at tasks like working together as a swarm to gather piles of iron oxide particles. And while the form and function of the xenobots was achieved without any genetic engineering, in an extra experiment the team injected them with RNA that caused them to produce a fluorescent protein that changes color when exposed to a particular color of light.
This allowed the xenobots to record whether they had come into contact with a specific light source while traveling about. The researchers say this is a proof of principle that the xenobots can be imbued with a molecular memory, and future work could allow them to record multiple stimuli and potentially even react to them.
What exactly these xenobots could eventually be used for is still speculative, but they have features that make them a promising alternative to non-organic alternatives. For a start, robots made of stem cells are completely biodegradable and also have their own power source in the form of “yolk platelets” found in all amphibian embryos. They are also able to self-heal in as little as five minutes if cut, and can take advantage of cells’ ability to process all kinds of chemicals.
That suggests they could have applications in everything from therapeutics to environmental engineering. But the researchers also hope to use them to better understand the processes that allow individual cells to combine and work together to create a larger organism, and how these processes might be harnessed and guided for regenerative medicine.
As these animal-machine hybrids advance, they are sure to raise ethical concerns and question marks over the potential risks. But it looks like the future of robotics could be a lot more wet and squishy than we imagined.
Image Credit: Doug Blackiston/Tufts University Continue reading
Posted in Human Robots
#439032 To Learn To Deal With Uncertainty, This ...
AI is endowing robots, autonomous vehicles and countless of other forms of tech with new abilities and levels of self-sufficiency. Yet these models faithfully “make decisions” based on whatever data is fed into them, which could have dangerous consequences. For instance, if an autonomous car is driving down a highway and the sensor picks up a confusing signal (e.g., a paint smudge that is incorrectly interpreted as a lane marking), this could cause the car to swerve into another lane unnecessarily.
But in the ever-evolving world of AI, researchers are developing new ways to address challenges like this. One group of researchers has devised a new algorithm that allows the AI model to account for uncertain data, which they describe in a study published February 15 in IEEE Transactions on Neural Networks and Learning Systems.
“While we would like robots to work seamlessly in the real world, the real world is full of uncertainty,” says Michael Everett, a post-doctoral associate at MIT who helped develop the new approach. “It's important for a system to be aware of what it knows and what it is unsure about, which has been a major challenge for modern AI.”
His team focused on a type of AI called reinforcement learning (RL), whereby the model tries to learn the “value” of taking each action in a given scenario through trial-and-error. They developed a secondary algorithm, called Certified Adversarial Robustness for deep RL (CARRL), that can be built on top of an existing RL model.
“Our key innovation is that rather than blindly trusting the measurements, as is done today [by AI models], our algorithm CARRL thinks through all possible measurements that could have been made, and makes a decision that considers the worst-case outcome,” explains Everett.
In their study, the researchers tested CARRL across several different tasks, including collision avoidance simulations and Atari pong. For younger readers who may not be familiar with it, Atari pong is a classic computer game whereby an electronic paddle is used to direct a ping pong on the screen. In the test scenario, CARRL helped move the paddle slightly higher or lower to compensate for the possibility that the ball could approach at a slightly different point than what the input data indicated. All the while, CARRL would try to ensure that the ball would make contact with at least some part of paddle.
Gif: MIT Aerospace Controls Laboratory
In a perfect world, the information that an AI model is fed would be accurate all the time and AI model will perform well (left). But in some cases, the AI may be given inaccurate data, causing it to miss its targets (middle). The new algorithm CARRL helps AIs account for uncertainty in its data inputs, yielding a better performance when relying on poor data (right).
Across all test scenarios, the RL model was better at compensating for potential inaccurate or “noisy” data with CARRL, than without CARRL.
But the results also show that, like with humans, too much self-doubt and uncertainty can be unhelpful. In the collision avoidance scenario, for example, indulging in too much uncertainty caused the main moving object in the simulation to avoid both the obstacle and its goal. “There is definitely a limit to how ‘skeptical’ the algorithm can be without becoming overly conservative,” Everett says.
This research was funded by Ford Motor Company, but Everett notes that it could be applicable under many other commercial applications requiring safety-aware AI, including aerospace, healthcare, or manufacturing domains.
“This work is a step toward my vision of creating ‘certifiable learning machines’—systems that can discover how to explore and perform in the real world on their own, while still having safety and robustness guarantees,” says Everett. “We'd like to bring CARRL into robotic hardware while continuing to explore the theoretical challenges at the interface of robotics and AI.” Continue reading
Posted in Human Robots
#439012 Video Friday: Man-Machine Synergy ...
Video Friday is your weekly selection of awesome robotics videos, collected by your Automaton bloggers. We’ll also be posting a weekly calendar of upcoming robotics events for the next few months; here's what we have so far (send us your events!):
RoboSoft 2021 – April 12-16, 2021 – [Online Conference]
ICRA 2021 – May 30-5, 2021 – Xi'an, China
DARPA SubT Finals – September 21-23, 2021 – Louisville, KY, USA
WeRobot 2021 – September 23-25, 2021 – Coral Gables, FL, USA
Let us know if you have suggestions for next week, and enjoy today's videos.
Man-Machine Synergy Effectors, Inc. is a Japanese company working on an absolutely massive “human machine synergistic effect device,” which is a huge robot controlled by a nearby human using a haptic rig.
From the look of things, the next generation will be able to move around. Whoa.
[ MMSE ]
This method of loading and unloading AMRs without having them ever stop moving is so obvious that there must be some equally obvious reason why I've never seen it done in practice.
The LoadRunner is able to transport and sort parcels weighing up to 30 kilograms. This makes it the perfect luggage carrier for airports. These AI-driven go-carts can also work in concert as larger collectives to carry large, heavy and bulky objects. Every LoadRunner can also haul up to four passive trailers. Powered by four electric motors, the LoadRunner sharply brakes at just the right moment right in front of its destination and the payload slides from the robot onto the delivery platform.
[ Fraunhofer ] via [ Gizmodo ]
Ayato Kanada at Kyushu University wrote in to share this clever “dislocatable joint,” a way of combining continuum and rigid robots.
[ Paper ]
Thanks Ayato!
The DodgeDrone challenge revisits the popular dodgeball game in the context of autonomous drones. Specifically, participants will have to code navigation policies to fly drones between waypoints while avoiding dynamic obstacles. Drones are fast but fragile systems: as soon as something hits them, they will crash! Since objects will move towards the drone with different speeds and acceleration, smart algorithms are required to avoid them!
This could totally happen in real life, and we need to be prepared for it!
[ DodgeDrone Challenge ]
In addition to winning the Best Student Design Competition CREATIVITY Award at HRI 2021, this paper would also have won the Best Paper Title award, if that award existed.
[ Paper ]
Robots are traditionally bound by a fixed morphology during their operational lifetime, which is limited to adapting only their control strategies. Here we present the first quadrupedal robot that can morphologically adapt to different environmental conditions in outdoor, unstructured environments.
We show that the robot exploits its training to effectively transition between different morphological configurations, exhibiting substantial performance improvements over a non-adaptive approach. The demonstrated benefits of real-world morphological adaptation demonstrate the potential for a new embodied way of incorporating adaptation into future robotic designs.
[ Nature ]
A drone video shot in a Minneapolis bowling alley was hailed as an instant classic. One Hollywood veteran said it “adds to the language and vocabulary of cinema.” One IEEE Spectrum editor said “hey that's pretty cool.”
[ Bryant Lake Bowl ]
It doesn't take a robot to convince me to buy candy, but I think if I buy candy from Relay it's a business expense, right?
[ RIS ]
DARPA is making progress on its AI dogfighting program, with physical flight tests expected this year.
[ DARPA ACE ]
Unitree Robotics has realized that the Empire needs to be overthrown!
[ Unitree ]
Windhover Labs, an emerging leader in open and reliable flight software and hardware, announces the upcoming availability of its first hardware product, a low cost modular flight computer for commercial drones and small satellites.
[ Windhover ]
As robots and autonomous systems are poised to become part of our everyday lives, the University of Michigan and Ford are opening a one-of-a-kind facility where they’ll develop robots and roboticists that help make lives better, keep people safer and build a more equitable society.
[ U Michigan ]
The adaptive robot Rizon combined with a new hybrid electrostatic and gecko-inspired gripping pad developed by Stanford BDML can manipulate bulky, non-smooth items in the most effort-saving way, which broadens the applications in retail and household environments.
[ Flexiv ]
Thanks Yunfan!
I don't know why anyone would want things to get MORE icy, but if you do for some reason, you can make it happen with a Husky.
Is winter over yet?
[ Clearpath ]
Skip ahead to about 1:20 to see a pair of Gita robots following a Spot following a human like a chain of lil’ robot duckings.
[ PFF ]
Here are a couple of retro robotics videos, one showing teleoperated humanoids from 2000, and the other showing a robotic guide dog from 1976 (!)
[ Tachi Lab ]
Thanks Fan!
If you missed Chad Jenkins' talk “That Ain’t Right: AI Mistakes and Black Lives” last time, here's another opportunity to watch from Robotics Today, and it includes a top notch panel discussion at the end.
[ Robotics Today ]
Since its founding in 1979, the Robotics Institute (RI) at Carnegie Mellon University has been leading the world in robotics research and education. In the mid 1990s, RI created NREC as the applied R&D center within the Institute with a specific mission to apply robotics technology in an impactful way on real-world applications. In this talk, I will go over numerous R&D programs that I have led at NREC in the past 25 years.
[ CMU ] Continue reading
Posted in Human Robots